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pcdna5 frt to mvenus 3xflag gateway  (Addgene inc)


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    Structured Review

    Addgene inc pcdna5 frt to mvenus 3xflag gateway
    Pcdna5 Frt To Mvenus 3xflag Gateway, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3xflag+pcdna5+frt/pcDNA5%2FFRT%2FTO-Venus-Flag-Gateway+(1124)+(Plasmid+%2340999)/pm41875887-911-6-7
    Average 92 stars, based on 10 article reviews
    pcdna5 frt to mvenus 3xflag gateway - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Generated:

    Article Title: Oncogenic STIL-mediated loss of BRCA1 functionality causes DNA damage and centrosome amplification
    Article Snippet: The pcDNA5 FRT/TO MycLAP-STIL plasmids was ordered from Addgene (#80266). .. The pcDNA5 FRT/TO/EGFP plasmids is a gift from the laboratory of Prof. Andrew Mussachio. pcDNA5 FRT/TO MycLAP-STIL_S76L and pcDNA5 FRT/TO MycLAP-STIL_S76L_S183A were generated using site-directed mutagenesis, using primers 5’-CAGAATAAAAAAAATTTGTCATGCTTTTTACTT-3’ and 5’-GAGAGTTTGGACGCTGTGGAATTT-3’ respectively. pDEST 3xFlag-pcDNA5-FRT/TO-BRCA1 was ordered from Addgene (#52504) Plasmids were transfected using Polyethylenimine Max (Polysciences #23966-100). .. Following primary antibodies for immunofluorescence (IF) and western blotting (WB) with indicated dilution were used: mouse anti-γ tubulin antibody (Sigma #T5326, 1:100 IF), rabbit anti-Centrin-1 antibody (Abcam#ab101332, 1:500 IF), mouse anti-Centrin-1 20H5 antibody (Merck Millipore#04-1624, 1:200 IF), mouse anti-α tubulin antibody (Sigma#T9026, 1:100 IF, 1:1000 WB), mouse anti-BRCA1 D-9 antibody (Santa Cruz Biotechnology#SC6954, 1:100 IF, 1:1000 WB), rabbit anti-STIL antibody (Abcam#ab89314, 1:50 IF, 1:2000 WB), rabbit anti-ubiquitin antibody (Abcam#ab134953, 1:2000 WB) and rabbit anti-GFP antibody (Sigma #G1544, 1:7000 WB).

    Mutagenesis:

    Article Title: Oncogenic STIL-mediated loss of BRCA1 functionality causes DNA damage and centrosome amplification
    Article Snippet: The pcDNA5 FRT/TO MycLAP-STIL plasmids was ordered from Addgene (#80266). .. The pcDNA5 FRT/TO/EGFP plasmids is a gift from the laboratory of Prof. Andrew Mussachio. pcDNA5 FRT/TO MycLAP-STIL_S76L and pcDNA5 FRT/TO MycLAP-STIL_S76L_S183A were generated using site-directed mutagenesis, using primers 5’-CAGAATAAAAAAAATTTGTCATGCTTTTTACTT-3’ and 5’-GAGAGTTTGGACGCTGTGGAATTT-3’ respectively. pDEST 3xFlag-pcDNA5-FRT/TO-BRCA1 was ordered from Addgene (#52504) Plasmids were transfected using Polyethylenimine Max (Polysciences #23966-100). .. Following primary antibodies for immunofluorescence (IF) and western blotting (WB) with indicated dilution were used: mouse anti-γ tubulin antibody (Sigma #T5326, 1:100 IF), rabbit anti-Centrin-1 antibody (Abcam#ab101332, 1:500 IF), mouse anti-Centrin-1 20H5 antibody (Merck Millipore#04-1624, 1:200 IF), mouse anti-α tubulin antibody (Sigma#T9026, 1:100 IF, 1:1000 WB), mouse anti-BRCA1 D-9 antibody (Santa Cruz Biotechnology#SC6954, 1:100 IF, 1:1000 WB), rabbit anti-STIL antibody (Abcam#ab89314, 1:50 IF, 1:2000 WB), rabbit anti-ubiquitin antibody (Abcam#ab134953, 1:2000 WB) and rabbit anti-GFP antibody (Sigma #G1544, 1:7000 WB).

    Transfection:

    Article Title: Oncogenic STIL-mediated loss of BRCA1 functionality causes DNA damage and centrosome amplification
    Article Snippet: The pcDNA5 FRT/TO MycLAP-STIL plasmids was ordered from Addgene (#80266). .. The pcDNA5 FRT/TO/EGFP plasmids is a gift from the laboratory of Prof. Andrew Mussachio. pcDNA5 FRT/TO MycLAP-STIL_S76L and pcDNA5 FRT/TO MycLAP-STIL_S76L_S183A were generated using site-directed mutagenesis, using primers 5’-CAGAATAAAAAAAATTTGTCATGCTTTTTACTT-3’ and 5’-GAGAGTTTGGACGCTGTGGAATTT-3’ respectively. pDEST 3xFlag-pcDNA5-FRT/TO-BRCA1 was ordered from Addgene (#52504) Plasmids were transfected using Polyethylenimine Max (Polysciences #23966-100). .. Following primary antibodies for immunofluorescence (IF) and western blotting (WB) with indicated dilution were used: mouse anti-γ tubulin antibody (Sigma #T5326, 1:100 IF), rabbit anti-Centrin-1 antibody (Abcam#ab101332, 1:500 IF), mouse anti-Centrin-1 20H5 antibody (Merck Millipore#04-1624, 1:200 IF), mouse anti-α tubulin antibody (Sigma#T9026, 1:100 IF, 1:1000 WB), mouse anti-BRCA1 D-9 antibody (Santa Cruz Biotechnology#SC6954, 1:100 IF, 1:1000 WB), rabbit anti-STIL antibody (Abcam#ab89314, 1:50 IF, 1:2000 WB), rabbit anti-ubiquitin antibody (Abcam#ab134953, 1:2000 WB) and rabbit anti-GFP antibody (Sigma #G1544, 1:7000 WB).



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